Fetal RHD and RHCE Genotyping in Plasma of Rh Negative Pregnant Women
Joana Oliveira, Nádia Osório, José Rocha, Benvinda Cruz, João Figueiredo, Armando Caseiro, Ana Valado, António Gabriel, Fernando Mendes
Int. J. Bio. Lab. Sci  2012  1:50-58 Abstract】 PDF
 

Abstract
Aim: To assess the accuracy of RHD and RHCE genotyping by real-time PCR, through the analysis of fetal DNA in plasma samples of Rh negative pregnant women.
Methods: The study consisted in the collection of two tubes of blood (each with 3 ml) from 19 RhD negative pregnant women that attended the obstetric consultation at the District Hospital of Figueira da Foz, E.P.E. One of the samples was used to perform blood typing and the other was used for fetal DNA extraction. The Rh genotype was determined by real-time PCR with specific primers and Taqman® probes for RHD, RHC, RHE, SRY and GLO genes.
Results: In 88.2% of cases (15/17) the RHD genotype was concordant with the phenotyping data, with 1 false-positive result (5.9%) and a precision of 94% (pvalue=0.001; K=76.7%). For the RHC gene, there was concordance in 60% of cases (6/10), with 4 false-negative results (40%) and a precision of 60% (pvalue=0,175; K=31%). Relatively to the RHE gene, the concordance achieved was
100% (10/10), with a precision of also 100% (pvalue=0.002; K=100%).
Conclusion: The present study confirms the precision of fetal RHD and RHE genotyping in maternal plasma. Performing a similar study with a superior number of samples could allow the implementation of this non-invasive prenatal diagnostic test in laboratorial and clinical routine, in order to follow pregnancies at risk for developing Hemolytic Disease of the Fetus and Newborn.
Key words: Fetal DNA, maternal plasma, Hemolytic Disease of the Fetus and Newborn, Anti-D prophylaxis, non-invasive prenatal diagnosis

Evaluation of Absolute Lymphocyte Count as a Surrogate marker for CD4+ cell count for the Initiation of Antiretroviral Therapy (ART) in Resource-limited Settings
F. I. Buseri, D. Mark, Z. A. Jeremiah
Int. J. Bio. Lab. Sci  2012  1:44-49 Abstract】 PDF
 

Abstract
Background: In resource-limited settings, laboratory assessment of HIV-infected individuals requires the consideration of simple markers for the initiation and monitoring of HAART. Absolute lymphocyte count (ALC) could serve as a simple cost-effective tool for determining when to initiate ART in resource-constrained settings.
Objective: To determine the possibility of using absolute lymphocyte count as a surrogate marker for CD4+ cell count for the initiation of antiretroviral therapy (ART) in resource-limited settings.
Methods: Blood samples from 273 symptomatic HIV-positive individuals aged 18 to 57 years (33.3% males, 66.7% females) who presented for assessment for ART-eligibility were analyzed for CD4 count by flow-cytometry and ALC by automated haematology cell counter. Spearman rank correlation between CD4 count and ALC was assessed and positive predictive value (PPV), negative
predictive value (NPV), sensitivity and specificity of varying ALC cutoffs were computed for CD4 counts <200 cells/μL and <350 cells/μL.
Results: High degree of correlation was noted between CD4 and ALC counts (r = 0.71, p<0.0001). PPV, NPV, sensitivity, and specificity maximally aggregated at ALC <1400 cells/μL for CD4 <200 cells/μL and ALC <2300 cells/μL for CD4 <350 cells/μL. ALC <1400 cells/μL had a 90% PPV, 87% NPV, 86% sensitivity and 90% specificity for CD4 counts <200 cells/μL. ALC <2300 cells/μL had 89% PPV, 51% NPV, 83% sensitivity and 62% specificity for CD4 counts <350 cells/μL.
Conclusion: Our results have demonstrated the capability of absolute lymphocyte count to serve as a surrogate marker for CD4+ cell count for the initiation of antiretroviral therapy (ART) in resource-limited settings.
Key words: Absolute lymphocyte count, CD4+ cell count, antiretroviral therapy, resource-limited settings

Total Antioxidant Status and other Antioxidant Agent Levels in Children with P.Falciparum Infection in Calabar, Nigeria
Akpotuzor J. O, Udoh A. E, Etukudo M. H
Int. J. Bio. Lab. Sci  2012  1:35-39 Abstract】 PDF
 

Abstract
Malaria infection and its treatment with antimalaria drugs have been reported to generate oxygen free radicals. These free radicals generated in the cause of infection and its treatment need to be removed from the body by reducing agents called antioxidants. This study is aimed at evaluating the impact of malaria infection on total antioxidant status of children. By using standard procedures
and kits purchased from Randox laboratories, USA and Quimica Clinica Aplicada Spain, total antioxidant level, vitamin C, Total and HDL cholesterol and albumin were assessed in 122 malaria infected children. The haemoglobin and parasite density status of the children were also measured. Sixty healthy children were used as controls. It was observed that all parameters measured except the total cholesterol (which was similar in both malaria infected children and the control
subjects), were significantly lower in malaria infected children when compared with the respective control values. Malaria parasitemia correlated strongly and negatively with total antioxidant levels (r= 0.-432).Reduction in the level of total antioxidant was dependent on the severity of malaria hence the more severe the malaria, the lower the level of total antioxidant. From this study it is observed that there is a general depression in total antioxidant levels as well as HDL Cholesterol, albumin and vitamin C levels suggesting increased consumption of antioxidants and its agents during malaria infection. Therefore, antioxidant intervention may be crucial in the treatment of malaria infection.
Key words: Antioxidant status, P. Falciparum infection, malaria, Vitamin C

Frequency of Blood Donation and Iron Stores of Blood Donors in Calabar, Cross River, Nigeria
Okpokam, D.C, Emeribe, A.O, Akpotuzor, J.O
Int. J. Bio. Lab. Sci  2012  1:40-43 Abstract】 PDF
 

Abstract
The purpose of this study was to assess the influence of frequency of blood donation on iron stores in 163 male blood donors. Forty one were 1st time (control group), Thirty-eight were 2nd time, thirty-eight were 3rd time, thirty one were 4th time and fifteen were 5th time in UCTH Calabar. Packed cell volume (PCV) and haemoglobin (Hb) concentration were determined using particle counter model PCE-210, while serum iron (SI) and total iron binding capacity (TIBC) were determined using kits purchased from Human Diagnostics (D-65205) Wiesbaden - Germany. Transferrin saturation (TS) was determined by computation of serum iron concentration and total iron binding capacity values while serum ferritin (SF) was determined using enzyme linked immunoassay method kit purchased from Boehringer Mannheim Immunodiagnostics (Cat No. BC -1025 and BC - 1120) from Germany. The PCV, Hb, SI, TIBC, TS and SF of 2nd, 3rd, 4th and 5th time blood donors were significantly reduced (P>0.001) progressively as frequency of donation increased when compared with the 1st time blood donors. However, their mean cell haemoglobin concentration (MCHC) revealed no significant change (P>0.001) as frequency of donation increased. A strong and significant positive correlation existed between SF and PCV, SF and Hb (P< 0.05). 10.5%, 41.9% and 60% of 3rd, 4th, and 5th time blood donors within the year respectively had deficicient iron stores with transferrin saturation of <16%. It is the conclusion of this study that increased frequency of blood donation reduced the iron stores of donors. Therefore, donors should be advised on eating balanced diet as well as the use of iron supplements.
Key words: Blood donors, iron stores, serum iron, transferrin saturation, serum ferritin

International Journal of Biomedical Laboratory Science(IJBLS) Vol. 1, No. 2:35-58
July, 2012
 

CONTENTS

Original Articles
► Total Antioxidant Status and other Antioxidant Agent Levels in Children with P.Falciparum Infection in Calabar, Nigeria
Akpotuzor J. O, Udoh A. E, Etukudo M. H
Int. J. Bio. Lab. Sci 2012 1:35-39 【Abstract】 【PDF】
 
► Frequency of Blood Donation and Iron Stores of Blood Donors in Calabar, Cross River, Nigeria
Okpokam, D.C, Emeribe, A.O, Akpotuzor, J.O
Int. J. Bio. Lab. Sci 2012 1:40-43 【Abstract】 【PDF】
 
► Evaluation of Absolute Lymphocyte Count as a Surrogate marker for CD4+ cell count for the Initiation of Antiretroviral Therapy (ART) in Resource-limited Settings
F. I. Buseri, D. Mark, Z. A. Jeremiah
Int. J. Bio. Lab. Sci 2012 1:44-49 【Abstract】 【PDF】
 
► Fetal RHD and RHCE Genotyping in Plasma of Rh Negative Pregnant Women
Joana Oliveira, Nádia Osório, José Rocha, Benvinda Cruz, João Figueiredo, Armando Caseiro, Ana Valado, António Gabriel, Fernando Mendes
Int. J. Bio. Lab. Sci 2012 1:50-58 【Abstract】 【PDF】
 

Published 【PDF】

2012 Vol.1. No2

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