Regulation of Metal Metalloproteinase 9 Expression and Activity
Yi-Ching Huang, Yih-Hsin Chang, Ming-Yuh Shiau
Int. J. Bio. Lab. Sci  2018  1&2:15-19 Abstract】 PDF
 

Abstract
Our previous study demonstrates that matrix metalloproteinase 2 (MMP-2) is differentially regulated by the interplay between glucose and insulin. In this context, it was intriguing to examine the regulation of the other well-studied MMPs, MMP-9, in 3T3-L1 pre-adipocytes. Our results revealed that, as the scenario in MMP-2 regulation, glucose was a pre-requisite for determining pre-adipocytes’ responses to external stimuli. Short-term and long-term insulin exposure exhibited opposite regulation to MMP-9 under high glucose-containing environment. The dominant MMP-9 regulatory role of long-term insulin under euglycemia was diminished when culture environment contained high glucose concentration. Our results suggest pre-adipocytes may participate in the process of increasing adiposity through mediating extracellular matrix alterations resulted from the changes of MMP-9 expression/activity.
Key words: insulin, glucose, obesity, pre-adipocytes, adipocytes, matrix metalloproteinase-9

Association of Bacteriuria with the Presence of Nitrite and Pus Cells
Zenoh Danjuma, Thumamo Pokam, Kashibu Emmanuel, Babylon Philemon, Yakubu Nosano
Int. J. Bio. Lab. Sci  2018  1&2:9-14 Abstract】 PDF
 

Abstract
This study attempts to investigate the level of association of bacteriuria with the presence of nitrite and pus cells in urine. Methodology: Over 220 subjects were examined; 20 mL of mid-stream urine was collected and cultured on CLED and Blood agar using a calibrated wire loop of 0.001 mL, then analyzed using combi 9 rapid test kits. About 5mL was centrifuge at 2000rpm for 5 minutes and the sediment was examined with a microscope. Results: Of the 220 subjects examined, 143(65%) yielded bacterial growth, 35(24%) with a count of ≤103 CFU/mL, 46(32%) with 104 CFU/mL, and 62(43%) had ≥105 CFU/mL. Nitrite was present in 54/220(26%) of the samples, and 43(80%) were culture positive; it has a sensitivity of 30% and specificity of 80. Pyuria was detected in 102/220(46%) samples, with 61(60%) yielding positive culture, the sensitivity and specificity were 43% and 60% respectively. Thirty-four (24%) of 143 subjects with positive culture, had both pyuria and nitrite in their urine, and 27(44%) and 7(21%) were positive for only pyuria and nitrite respectively. Statistical analysis showed a lack significant association between; bacteriuria and the presence of nitrite (P=0.13); between pyuria and bacteriuria (P=0.064). The occurrence of both pyuria and nitrite, was strongly linked to bacteriuria (P=0.0003). Conclusion: The presence of both pus cells and nitrite in urine signifies UTIs, but the occurrence of only one of the parameters are not reliable, and the absence of anyone or both parameters does not rule out UTIs.
Key words: Urinary Tract Infection (UTIs), Pyuria, Nitrite, Bacteriuria, mid-stream urine, Colony forming unit (CFU/mL)

International Journal of Biomedical Laboratory Science(IJBLS) Vol. 7, No. 1&2:1-19
December, 2018

 

CONTENTS

Minireview
► Developing Educational Content about the Preanalytical Phase of the Total Testing Process for Non-laboratory Healthcare Personnel
Holappa-Girginkaya, Jaana, Mäkitalo, Outi
Int. J. Bio. Lab. Sci 2018 1&2:1-8 【Abstract】 【PDF】 
 
Original Articles
► Association of Bacteriuria with the Presence of Nitrite and Pus Cells
Zenoh Danjuma, Thumamo Pokam, Kashibu Emmanuel, Babylon Philemon, Yakubu Nosano
Int. J. Bio. Lab. Sci 2018 1&2:9-14 【Abstract】 【PDF】 
 
►Regulation of Metal Metalloproteinase 9 Expression and Activity
Yi-Ching Huang, Yih-Hsin Chang, Ming-Yuh Shiau
Int. J. Bio. Lab. Sci 2018 1&2:15-19 【Abstract】 【PDF】
 

Published 【PDF】

 2018 Vol. 7 No.1 2

Developing Educational Content about the Preanalytical Phase of the Total Testing Process for Non-laboratory Healthcare Personnel
Holappa-Girginkaya, Jaana, Mäkitalo, Outi
Int. J. Bio. Lab. Sci  2018  1&2:1-8 Abstract】 PDF
 

Abstract
Educational content is essential for shaping and advancing knowledge on, and improving the quality of, the preanalytical phase of the total testing process for biological samples. Process steps occurring after the preanalytical phase are also impacted by educational content about this phase. This literature review includes studies on education and training that focus on blood collection performed by non-laboratory healthcare staff. The aim is to suggest developments for educational content on sample collection occurring outside the laboratory to facilitate learning and improve the quality of the preanalytical phase of the total testing process when performed by non-laboratory healthcare staff. The 39 articles included in this review were published from 2010 to 2016, and were identified from an initial literature search of electronic databases conducted in 2015, and a second additional search conducted at the end of 2016 using keywords and certain selection criteria. The articles were categorized into three groups according to the suggestions for educational content for training non-laboratory staff about the preanalytical phase of the total testing process. Each of three categories represented an approach to the content that could influence knowledge development and the quality of the training. The findings of this review provide new and unconventional perspectives that may be used to further develop educational content for, and the knowledge of, non-laboratory healthcare professionals regarding biological sample collection. The findings also contribute to reduced field errors by non-laboratory healthcare professionals during the preanalytical phase of the total testing process.
Key words: literature review, preanalytical, blood sample collection, education, multi-professional

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