Salivary and Serum Matrix Metalloproteinase (MMP)-2, MMP-9 and Their Tissue Inhibitors (TIMP)-1 and TIMP-2 in Young Obesity
Fréderic Mota, Ana Filipa Silva, Daniela Almeida, Carla Oliveira, Tiago Luís, Carlos Tavares, João Paulo Figueiredo, Rui Gonçalves, e Armando Caseiro
Int. J. Bio. Lab. Sci 2019 1&2:10-21 【Abstract】 【PDF】
Objective: To assess the levels of MMP-9, MMP-2, and TIMP-1 and TIMP-2, and the MMP-9/TIMP-1, MMP-2/TIMP-2 ratios in serum and saliva in young individuals with obesity without comorbidities. Methods: 24 people with obesity, 24 overweight and 24 normal weight individuals were studied and grouped by body fat mass percentage by bioelectrical impedance analysis (BIA). Salivary and serum MMPs and TIMPs levels were measured by slot-blot analysis. Results: Individuals with obesity and overweight had higher MMP-9 levels in saliva comparing to normal weight (p ˂0.01 and p ˂0.05 respectively). People with obesity had higher MMP-2 levels than normal weight in saliva (p ˂0.05). Overweight had lower TIMP-1 levels in saliva than normal weight (p ˂0.05). We found higher net MMP-9 activity (higher MMP-9/TIMP-1 ratio) in saliva (p ˂0.01) and higher activity of MMP-2 (MMP-2/TIMP-2 ratio) in serum in overweight comparing to normal weight (p ˂0.05). Overall, no significant differences were found in serum results. Conclusion: Obesity is a chronic inflammatory disorder early associated with dysregulated MMP-9 and MMP-2 activity affecting oral and systemic health.
Key words: Obesity, Matrix Metalloproteinases, Saliva, Inflammation